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pet15b expression vector  (Addgene inc)


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    Structured Review

    Addgene inc pet15b expression vector
    Pet15b Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet15b+vector/SP-Cas9+(Plasmid+%2362731)/pmc12901986-317-11-14
    Average 96 stars, based on 369 article reviews
    pet15b expression vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title:
    Article Snippet: .. A pET15b vector encoding the catalytic subunit α of Protein Kinase A was purchased from Addgene (Addgene plasmid 14921) (25). .. The plasmid was transformed into Escherichia coli strain BL21-CodonPlus (DE3)-RIL (Stratagene) and protein expression was carried out in LB after induction with 1 mM IPTG.

    Article Title: Intracellular FGF1 protects cells from apoptosis through direct interaction with p53.
    Article Snippet: For cell transfection experiments, sequences encoding N-terminal myc- or SBP-tagged FGF1 were cloned into the pcDNA3.1 vector (Invitrogen, CA, USA). .. Constructs encoding the his-tagged full-length p53 (p531−393) and the p53 DNA-binding domain (p53_DBD: p5394−312) in the pET15b vector for bacterial expression were obtained from Cheryl Arrowsmith via Addgene (plasmid #24859 and #24866) [21]. .. The wild type hBax C3-EGFP (Bax-EGFP) construct was provided by Richard Youle through Addgene (plasmid #19741) [22].

    Article Title: N-Terminal Ubiquitination of Amyloidogenic Proteins Triggers Removal of Their Oligomers by the Proteasome Holoenzyme
    Article Snippet: .. The full-length sequence of mouse E1 in pET28a vector (kind gift from David Komander) and human UBE2W in pET15b vector (kind gift from Wade Harper, Addgene plasmid #15809) were used for protein expression. ..

    Article Title: Phosphorylated full-length Tau interacts with 14-3-3 proteins via two short phosphorylated sequences, each occupying a binding groove of 14-3-3 dimer.
    Article Snippet: .. Protein Kinase A catalytic subunit production and purification E. coli BL21 (DE3) cells were transformed with the pet15b Vector carrying the cDNA coding the N-terminally His6-tagged PKA catalytic subunit α from Mus musculus (PKAc) (Addgene plasmid # 14921). ..

    Article Title: Intracellular FGF1 protects cells from apoptosis through direct interaction with p53
    Article Snippet: For cell transfection experiments, sequences encoding N-terminal myc- or SBP-tagged FGF1 were cloned into the pcDNA3.1 vector (Invitrogen, CA, USA). .. Constructs encoding the his-tagged full-length p53 (p53 1−393 ) and the p53 DNA-binding domain (p53_DBD: p53 94−312 ) in the pET15b vector for bacterial expression were obtained from Cheryl Arrowsmith via Addgene (plasmid #24859 and #24866) [ ]. .. The wild type hBax C3-EGFP (Bax-EGFP) construct was provided by Richard Youle through Addgene (plasmid #19741) [ ].

    Article Title: Bright bioluminescent BRET sensor proteins for measuring intracellular caspase activity
    Article Snippet: .. ....................................................................................................................................... 7 References ...................................................................................................................................... 7 S‐2 Material & Methods Protein Expression and Purification All sensor constructs and caspase‐3 and ‐9(delta CARD) are encoded in a pET28a vector, while caspase‐8 was incorporated in a pET15b vector (pET15b‐Casp‐8 delta DED was a gift from Guy Salvesen (Addgene plasmid #11827).1 Sensors and proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) in 2 litres of LB medium. .. Expression was induced at an optical density (OD600) of ~0.6 by the addition of 0.5 mM isopropyl‐β‐ D‐thiogalactopyranoside (IPTG, AppliChem GmbH), followed by incubation overnight at 18 °C.

    Construct:

    Article Title: Intracellular FGF1 protects cells from apoptosis through direct interaction with p53.
    Article Snippet: For cell transfection experiments, sequences encoding N-terminal myc- or SBP-tagged FGF1 were cloned into the pcDNA3.1 vector (Invitrogen, CA, USA). .. Constructs encoding the his-tagged full-length p53 (p531−393) and the p53 DNA-binding domain (p53_DBD: p5394−312) in the pET15b vector for bacterial expression were obtained from Cheryl Arrowsmith via Addgene (plasmid #24859 and #24866) [21]. .. The wild type hBax C3-EGFP (Bax-EGFP) construct was provided by Richard Youle through Addgene (plasmid #19741) [22].

    Article Title: Intracellular FGF1 protects cells from apoptosis through direct interaction with p53
    Article Snippet: For cell transfection experiments, sequences encoding N-terminal myc- or SBP-tagged FGF1 were cloned into the pcDNA3.1 vector (Invitrogen, CA, USA). .. Constructs encoding the his-tagged full-length p53 (p53 1−393 ) and the p53 DNA-binding domain (p53_DBD: p53 94−312 ) in the pET15b vector for bacterial expression were obtained from Cheryl Arrowsmith via Addgene (plasmid #24859 and #24866) [ ]. .. The wild type hBax C3-EGFP (Bax-EGFP) construct was provided by Richard Youle through Addgene (plasmid #19741) [ ].

    Article Title: Bright bioluminescent BRET sensor proteins for measuring intracellular caspase activity
    Article Snippet: .. ....................................................................................................................................... 7 References ...................................................................................................................................... 7 S‐2 Material & Methods Protein Expression and Purification All sensor constructs and caspase‐3 and ‐9(delta CARD) are encoded in a pET28a vector, while caspase‐8 was incorporated in a pET15b vector (pET15b‐Casp‐8 delta DED was a gift from Guy Salvesen (Addgene plasmid #11827).1 Sensors and proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) in 2 litres of LB medium. .. Expression was induced at an optical density (OD600) of ~0.6 by the addition of 0.5 mM isopropyl‐β‐ D‐thiogalactopyranoside (IPTG, AppliChem GmbH), followed by incubation overnight at 18 °C.

    Expressing:

    Article Title: Intracellular FGF1 protects cells from apoptosis through direct interaction with p53.
    Article Snippet: For cell transfection experiments, sequences encoding N-terminal myc- or SBP-tagged FGF1 were cloned into the pcDNA3.1 vector (Invitrogen, CA, USA). .. Constructs encoding the his-tagged full-length p53 (p531−393) and the p53 DNA-binding domain (p53_DBD: p5394−312) in the pET15b vector for bacterial expression were obtained from Cheryl Arrowsmith via Addgene (plasmid #24859 and #24866) [21]. .. The wild type hBax C3-EGFP (Bax-EGFP) construct was provided by Richard Youle through Addgene (plasmid #19741) [22].

    Article Title: N-Terminal Ubiquitination of Amyloidogenic Proteins Triggers Removal of Their Oligomers by the Proteasome Holoenzyme
    Article Snippet: .. The full-length sequence of mouse E1 in pET28a vector (kind gift from David Komander) and human UBE2W in pET15b vector (kind gift from Wade Harper, Addgene plasmid #15809) were used for protein expression. ..

    Article Title: Intracellular FGF1 protects cells from apoptosis through direct interaction with p53
    Article Snippet: For cell transfection experiments, sequences encoding N-terminal myc- or SBP-tagged FGF1 were cloned into the pcDNA3.1 vector (Invitrogen, CA, USA). .. Constructs encoding the his-tagged full-length p53 (p53 1−393 ) and the p53 DNA-binding domain (p53_DBD: p53 94−312 ) in the pET15b vector for bacterial expression were obtained from Cheryl Arrowsmith via Addgene (plasmid #24859 and #24866) [ ]. .. The wild type hBax C3-EGFP (Bax-EGFP) construct was provided by Richard Youle through Addgene (plasmid #19741) [ ].

    Article Title: Bright bioluminescent BRET sensor proteins for measuring intracellular caspase activity
    Article Snippet: .. ....................................................................................................................................... 7 References ...................................................................................................................................... 7 S‐2 Material & Methods Protein Expression and Purification All sensor constructs and caspase‐3 and ‐9(delta CARD) are encoded in a pET28a vector, while caspase‐8 was incorporated in a pET15b vector (pET15b‐Casp‐8 delta DED was a gift from Guy Salvesen (Addgene plasmid #11827).1 Sensors and proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) in 2 litres of LB medium. .. Expression was induced at an optical density (OD600) of ~0.6 by the addition of 0.5 mM isopropyl‐β‐ D‐thiogalactopyranoside (IPTG, AppliChem GmbH), followed by incubation overnight at 18 °C.

    Recombinant:

    Article Title: Host cell p53 associates with the feline calicivirus major viral capsid protein VP1, the protease-polymerase NS6/7, and the double-stranded RNA playing a role in virus replication
    Article Snippet: .. Homo sapiens p53 wild type recombinant protein was obtained by electroelution from the E. coli BL21 (DE3) pLysS strain prokaryotic system using the pET15b vector [Human p53 (1–393), Cat. 24,859, Addgene]. .. Feline calicivirus VP1 Urbana strain recombinant protein was obtained from the pGEX-VP1 expression vector (Paredes-Morales et al.; unpublished data).

    Sequencing:

    Article Title: N-Terminal Ubiquitination of Amyloidogenic Proteins Triggers Removal of Their Oligomers by the Proteasome Holoenzyme
    Article Snippet: .. The full-length sequence of mouse E1 in pET28a vector (kind gift from David Komander) and human UBE2W in pET15b vector (kind gift from Wade Harper, Addgene plasmid #15809) were used for protein expression. ..

    Purification:

    Article Title: Phosphorylated full-length Tau interacts with 14-3-3 proteins via two short phosphorylated sequences, each occupying a binding groove of 14-3-3 dimer.
    Article Snippet: .. Protein Kinase A catalytic subunit production and purification E. coli BL21 (DE3) cells were transformed with the pet15b Vector carrying the cDNA coding the N-terminally His6-tagged PKA catalytic subunit α from Mus musculus (PKAc) (Addgene plasmid # 14921). ..

    Article Title: Bright bioluminescent BRET sensor proteins for measuring intracellular caspase activity
    Article Snippet: .. ....................................................................................................................................... 7 References ...................................................................................................................................... 7 S‐2 Material & Methods Protein Expression and Purification All sensor constructs and caspase‐3 and ‐9(delta CARD) are encoded in a pET28a vector, while caspase‐8 was incorporated in a pET15b vector (pET15b‐Casp‐8 delta DED was a gift from Guy Salvesen (Addgene plasmid #11827).1 Sensors and proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) in 2 litres of LB medium. .. Expression was induced at an optical density (OD600) of ~0.6 by the addition of 0.5 mM isopropyl‐β‐ D‐thiogalactopyranoside (IPTG, AppliChem GmbH), followed by incubation overnight at 18 °C.

    Transformation Assay:

    Article Title: Phosphorylated full-length Tau interacts with 14-3-3 proteins via two short phosphorylated sequences, each occupying a binding groove of 14-3-3 dimer.
    Article Snippet: .. Protein Kinase A catalytic subunit production and purification E. coli BL21 (DE3) cells were transformed with the pet15b Vector carrying the cDNA coding the N-terminally His6-tagged PKA catalytic subunit α from Mus musculus (PKAc) (Addgene plasmid # 14921). ..



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